Live-cell imaging of NET formation in two different channels for up to 120 minutes. SYTOX Green at 10 nM was used for NET detection (depicted in green; left panels), and a TYE™-665 labeled hsa-miR142-3p locked nucleic acid (LNA) detection probe at 5 μM for miR-142-3p detection (depicted in red; middle panels). The hsa-miR-142-3p staining was present in two different morphological patterns: one shows a strong staining (asterisk), and the other reveals a weak staining that can be either punctate (blue arrow) or diffuse (white arrows). The right panels show the merge of the two channels. Bars: 20 μm. (Source: Linhares-Lacerda et al., 2020)

International Union of Immunological SocietiesUniversity of South AfricaInstitute of Infectious Disease and Molecular MedicineElizabeth Glazer Pediatric Aids FoundationStellenbosch University